Method for Isolation, Enumeration and Confirmation of Sulphite Reducing Clostridia and Clostridium perfringens from Raw, Potable, Process and Recreational Waters by Membrane Filtration

SOP: M/SOP07

The sample is filtered and the membrane filter placed on the surface of an agar medium containing sulphite and iron(III).  The agar medium is then incubated under anaerobic conditions at either  37°C for sulphite-reducing clostridia or 44°C for Clostridium perfringens.

Methodology – Environment Agency, The Microbiology of Drinking Water (2010), Part 6 – Methods for the isolation and enumeration of sulphite-reducing clostridia and Clostridium perfringens by membrane filtration.

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A number of different publications and standards cite sulphate methods. BR279 (1995) has largely been superseded by analytical procedures as detailed in TRL 447 and BRE Special Digest:1 2005 3rd Edition. In addition further reference may be made to BS EN 144-1:2009 + A1:2012 for the testing of aggregates and BS 1377:2018 for soils for civil engineering purposes.

Testing to meet the requirements of UKWIR Report Ref. No. 10/WM/03/21 Guidance for the selection of water supply pipes to be used in brownfield sites.

Testing & reporting is undertaken in accordance with BS 3882:2015 Specification for topsoil and BS 8601:2013 Specification for subsoil and requirements for use

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