Method for Isolation, Enumeration and Confirmation of Pseudomonas aeruginosa in Raw, Potable, Process and Recreational Waters by Membrane Filtration

SOP: M/SOP05

UKAS accredited

Pseudomonas aeruginosa are Gram negative, non-spore forming, oxidase positive bacilli which, in the context of this method, usually produce pyocyanin and fluorescin and are capable of hydrolysing casein.

Methodology – Environment Agency, The Microbiology of Drinking Water (2010), Part 8 – Methods for the isolation and enumeration of Aeromonas and Pseudomonas aeruginosa by membrane filtration.

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A number of different publications and standards cite sulphate methods. BR279 (1995) has largely been superseded by analytical procedures as detailed in TRL 447 and BRE Special Digest:1 2005 3rd Edition. In addition further reference may be made to BS EN 144-1:2009 + A1:2012 for the testing of aggregates and BS 1377:2018 for soils for civil engineering purposes.

Testing to meet the requirements of UKWIR Report Ref. No. 10/WM/03/21 Guidance for the selection of water supply pipes to be used in brownfield sites.

Testing & reporting is undertaken in accordance with BS 3882:2015 Specification for topsoil and BS 8601:2013 Specification for subsoil and requirements for use

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