Method for Isolation, Enumeration and Confirmation of Coliform and E.coli Organisms from Raw, Potable, Process and Recreational Waters by Membrane Filtration

SOP: M/SOP03

UKAS accredited

Presumptive coliform colonies are recognised by their yellow colour and E.coli colonies are recognised by their green colour. The yellow colouration is caused by the fermentation of lactose present in the membrane lactose glucuronide agar (MLGA) medium.

Methodology – The Microbiology of Drinking Water (2009) – Part 4- Methods for the isolation and enumeration of coliform bacteria and Escherichia coli.
Methods for the Examination of Waters and Associated Materials

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A number of different publications and standards cite sulphate methods. BR279 (1995) has largely been superseded by analytical procedures as detailed in TRL 447 and BRE Special Digest:1 2005 3rd Edition. In addition further reference may be made to BS EN 144-1:2009 + A1:2012 for the testing of aggregates and BS 1377:2018 for soils for civil engineering purposes.

Testing to meet the requirements of UKWIR Report Ref. No. 10/WM/03/21 Guidance for the selection of water supply pipes to be used in brownfield sites.

Testing & reporting is undertaken in accordance with BS 3882:2015 Specification for topsoil and BS 8601:2013 Specification for subsoil and requirements for use

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